Associate Professor at the University of Michigan. I frequently post photos of my cat and garden, and helpful tips for running a lab.
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2026-09-03T00:09:34Z Event JSON
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Last Notes npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab I agree that the impact on humans through blood donation may be overstated. npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab Chocolate eclairs may have gone out of style in the us, but maybe it’s just that I moved from the west coast where every corner has a boutique cafe to the Midwest where all we have are chains. npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab I really want to know who is giving students the uninformative template without telling them that they are actually supposed to replace the generic bullets words with their own. npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab See- npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab For example, this slide. npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab This slide is so useless. npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab Taking advantage of the bitter cold to freeze croquettes. Normally they stick together because I don’t have space in my freezer to freeze them on a cookie sheet. npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab Yeah for me it was how many electronics do I need to travel with. Work computer, personal tablet, phone, converters, and an ereader plus sketch pad, art pencils, etc. npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab In the end, I switched to reading on my tablet. It's heavier than I want and the battery isn't as long as an ereader, but it's fewer devices to lug around. npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab Factoid fact: npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab Bringing my potted peppers in this fall, I tried something different. npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab I decided to display those painting cards my sister in law gave me and I painted. A joyful daily reminder of one of my favorite things about my husband, his loving and welcoming family. npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab FFS-someone in the larger lab area is very sick, with a deep, congested cough, and they look like poop warmed over, and of course, they are wearing a paper mask around their chin. npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab A story in two parts. npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab You might be able to find a pre-made sambar mix if you don't have all of the sundry spices and tamarind. npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab Do you like Indian flavors? npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab What other tips or inspirations do people have to start a morning writing routine for scientists? npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab I think the first thing in the morning is best time to do this because I usually have a cup of coffee at work, so I can't do lab work anyway. I also rarely have meeting first thing in the morning. npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab So, I'm a New Year's resolution person. It's a way to try out new habits; sometimes they work, sometimes they don't. I'm ok with it not working, but I want to give it my best shot. npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab The stains on the chair are muddy kitty cat footprints because this is the chair under the window that kitty comes in and out of during the summer months when she comes in after we go to bed. npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab So unless you are going to beat the heck out of chairs, you can probably pick any heavy-weight fabric of a pattern you like and it'll last quite some time. npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab I've had similar chairs. I covered one with a heavy upholstery brocade, I really liked it. The other set my step mom covered in a striped fabric that I thought was too lightweight for chairs, I inherited the chairs when they moved to a different house and bought a new dining set. npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab Just once and the coffee was almost gone. npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab How long until I wet my brushes in the coffee (blue mug) versus water (clear cup)? npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab I gave myself a nice set of pens and a watercolor journal. This is slightly less fun because they don’t come out as well. Yesterday’s mushrooms were good, but today’s creature did not come out as well as the concept sketch. npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab Holiday fun. npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab In the Pacific Northwest? Want to help farmers operate more efficiently? Have experience in agricultural sales or related areas? Looking for a new job? npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab I’d like to find something to do with lime and lemon peels. Any favorite ideas? npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab I’m looking for game recommendations for an iPad. npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab I hope I’m not insufferable. npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab I may be a know it all. Twice in the same day, ppl exclaimed that I must be really good at trivia. The first was at a work event where I said something (can’t remember what) and one of my colleagues said it, the second was in my Pilates class. First I had cracked up when the Pilates instructor said something about the birch boxes being heavy because birch isn’t a heavy wood which I mentioned. Then later, she said Robert Palmer sang she’s got legs and I asked, wasn’t that ZZ Top. npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab Yup, not sure that happened. npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab Discovered that my host did it traditional with stuffing inside, because there was a lot of stuffing still in the carcass. npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab Making stock from the turkey carcass i snagged from Thanksgiving dinner and veggie scraps from the freezer. There were leek greens and OMG the stock smells so amazing. npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab 'Gardening' still going, pots in my make-do green house still pumping out greens and carrots. Overwintering peppers still giving some peppers. npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab Now is an excellent time to donate gently used toys or electronics so that they are available before the holidays. npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab Dang, I should get together 20 of my best buds and go in together on 55gal olive oil. npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab Pretty cool, every human protein predicted with every human protein. A couple of surprises for my favorite proteins. npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab Thanks! I am looking for an authority, there's lots of unpublished anecdotes or published things that are not quite right particularly in the endosome field. npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab Hey Sciencers: npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab Amazing eulogy for some reviled scientist by Harmit Malik on Bsky npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab That being said, I think if it hits tootles for me as a PI, I'll probably shift completely out of academia and science. Unless, I land some admin position at UM. I have applied for a few, but no dice. npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab Yes, this is why I gnash my teeth about all those people saying that the ECRs are getting such a bum deal trying to start their careers now. npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab So...although admissions is going to be way down in my field this year, we expect international applications to be way down. npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab I mean yes they do know. It's fricking brutal to be a scientist right now. npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab Ooof-had a scheduled chat with a supervisor about my funding (or lack thereof), and basically advised I should find another career path. Something along the lines of 'You've been struggling with funding for so long, even if you get this grant, its still going to be struggle. That's not the life he would want for me, is that the life you want for you?' npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab I got a relatively hefty Holiday Gift catalog in the mail this week. For whatever reason, I thought it was from Target. Turns out it was from Amazon. It was addressed to resident. npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab Confession/lab hack- npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab Oh my gawd. npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab PSA- don't fear the. colonoscopy npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab A slim majority voted for this, ~7,000 out of ~14,000 registered voters. npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab This is my first one. npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab Just ate the saddest side salad with lunch. npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab Oh dawg! I just remembered I live just outside of Detroit! I’ve totally been downtown several times. npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab Wow. Based on some talking points I've seen, I realize that I'm such a brave bad-ass or maybe blessed. I've been to DC three times. npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab More mushroom mayhem npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab #mushroom mayhem continues npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab Dinner! npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab Maybe it’s not water. Many liquids do not evaporate. Like the liquid that forms in a damp rid container that stuff stays liquid for months. npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab I discovered that in Michigan you can get a doctors note for illegally dark windows, like if you have glaucoma. npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab Dear Driver in the car with the illegally dark tinted windows, npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab Someone was saying tomato skins have a lot of bacteria so they should be removed. Maybe that’s the same ides npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab Fall mushroom season is here!! npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab Henceforth, I will call condensates jelly. npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab Self seeded anise hyssop being the star of fall. npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab I stopped trying to grow carrots in the ground followed @npub1fqf…ajkk advice(I think) and grew them in pots. Fantastic!! npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab Going to have some lions mane #mushrooms in a day or so. npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab #garden haul. npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab Question-someone who communicates on behalf of my department constantly uses the word ‘shall’ in professional emails non sarcastically. Eg I shall file this paperwork, I shall schedule this meeting. Basically using shall in place of will. npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab #Gardening question npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab I noticed that the brassicas in the back garden are much less moth eaten than the ones in the side garden. npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab In other news, I commented politely on the lack of alt text on posts coming from a very popular science account on bsky (22.5K followers). The poster quietly started adding alt text to their posts. npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab More importantly, we can suppress all of the defects caused by loss of Rab35 by overexpressing Rab7, the Rab that controls transport to the late endosome and lysosome. This strongly suggests that the massive expansion of endo-lysosomal system is fundamental for the establishment of the apicosome, and therefore needed to establish the lumen. 9/ npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab So, what is Rab35 doing for apicosome formation? We noticed that the massive lysosome does not form in cells lacking Rab35. Does that mean Rab35 is diverting excess apical proteins to the lysosome? We believe so. In cells lacking Rab35, we see higher levels of apical proteins by western blot, which could mean a defect in traffic to the lysosome. 8/ npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab So, this Rab35 localization is intriguing, but is Rab35 important? Yes. When we get rid of Rab35, we see dramatic effects on the formation of the apicosome. Instead of a single apicosome full of apical markers, we find that the apical markers accumulate multiple smaller structures, and primary cilium formation is disrupted. 7/ npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab We found that Rab35 is on the enlarged hybrid endosome (arrowheads) but not on the enlarged lysosome (asterisk). As the process concludes, Rab35 is found exclusively on the mature apicosome. This is different from its localization in most situations, where it resides on endosomes and promotes recycling. In addition, Rab35 is the only Rab we’ve found so far on the mature apicosome. 6/ npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab To get mechanistic insight into these changes, we used spatial proteomics to identify proteins near the apical protein podocalyxin, which is an important player in lumen formation. Here we identified a lot of endo-lysosomal protein including the Rab-GTPase Rab35. 5/ npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab We discovered that there is a massive, but transient enlargement of the endosomes. We find a >1uM hybrid endosome that contains markers of both the early and late endosome (arrowheads). In addition, there is an even larger >2uM late-endosome/lysosome (asterisk). These structures ultimately resolve into normal sized endosomes and lysosomes, surrounding the apicosome that we previously showed will give rise to the lumen. npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab In the epiblast, lumen formation initiates from unique organelle called the apicosome that contains apical proteins like podocalyxin and a primary cilium. But how does the apicosomes form? We knew that the apicosome was closely associated with various types of endosomes and also with lysosomes, and that it contains endocytosed material. Here, we explored the involvement of endosomes and lysosomes in the formation of the apicosome in a cellular model of early development. 3/ npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab You may recall from intro bio that lumens are very important in organogenesis. What you may not know is that there are many different ways to make a lumen. Here we explored the poorly understood type of lumen formation that occurs during epiblast formation an early stage in mammalian development. 2/ npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab I'm pleased to share collaborative work with @taniguchi-lab.bsky.social led by amazing graduate student Anusha. Here, we reveal unexpected dynamics in the endolysosomal system regulated by Rab35 that are critical for lumen formation in a model of early development. npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab The article I read said tourist visas too. npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab I wonder if it’s like the sort of insurance the EU demands of visitors from certain countries, npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab Hey Life Science folks, npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab Please applaud. I got carded buying nonalcoholic beer. I am 52. npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab Cool thanks! I only have a handful of go-to techniques and like to know what other people do. npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab Love this. What tool did you use for the darker features on the back? Sponge? npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab The year we had yellowjackets nest in our garden was one of the bests. They remove nearly every grub and caterpillar from the garden. npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab Next topic, the yard looks different have you added something? npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab Winter lettuce is easier that way. I only get aphids if I overwinter something and it brings in aphids in the fall. npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab The main problem is keeping them aphid free. We’re bringing in harvests from the yard and I know from past experience once the aphids take root I have to shutter operations for a few weeks to clear them out of the grow zone. npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab I’m doing some indoor lettuce and will try radishes but I’m not sure the grow light is strong enough for radishes. npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab Wait is this for now or for fall? npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab In-laws visiting for the first time since 2018. So Molly the cat is a new addition to the house for them. They’re from a generation where cats were not pets and dogs got leftovers of rice and lentils rather than dog food. npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab I think we’ve got a monarch in the milkweed. npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab Also the three line beetles like anise hyssop, that’s why we grow it on the deck. npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab I tried lures one year and caught a lot of beetles but didn’t see much change in the damage. I read online that the lures bring them in from far away. npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab Glad I left these one more day! npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab They survived I’m going to let them go one more day. npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 MCDuncanLab Periodic reminder.
