cityplus

So electrum personal server doesn’t work anymore unless you fix the certificates, which seems easy enough. But are there alternatives people are using?

2023-04-16 19:32:18 GMT

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Why is saturated fat bad?

2023-04-14 12:06:33 GMT

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Aeropress is the best!

2023-04-14 01:08:29 GMT

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Eth unstaking 😂

2023-04-14 00:18:19 GMT

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https://nostr.build/i/nostr.build_248133ab9f158bacecb17aca5122739769b99145e6b325f09bdae4e6d6aed309.png

2023-04-14 00:17:53 GMT

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Curious if you can explain what happened here?

2023-04-13 17:27:37 GMT

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No, not at all. I just wanted to clarify that you were criminalizing those who would use mRNA for illegitimate purposes not the mRNA itself. I find that many people dismiss the promise of mRNA because it has been used for the vaccine, which is a logical fallacy.

2023-04-13 17:25:58 GMT

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I have never seen data to support that “broken mRNA” is carcinogenic. That aside: therapeutics are always about risk and reward. When we are discussing terminal cancers and incurable genetic diseases, the risk of carcinogenicity may be justified (if it even exists).
I am suggesting that you not criminalize mRNA because it was used to make a vaccine that was rushed, unnecessary, or whatever you think it may be.

2023-04-13 14:29:55 GMT

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Why is mRNA stupid? Because it enabled vaccines? What if mRNA could also cure a previously incurable genetic condition?

2023-04-13 14:27:00 GMT

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No, I’m asking you why you posted the original note.

2023-04-13 14:07:08 GMT

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Of course, but how does this fit into the context of the original note?

2023-04-13 13:42:05 GMT

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Is your opinion that mRNA shouldn’t be pursued as a treatment for an intractable or terminal disease or incurable genetic condition because it could also be used for vaccines?

2023-04-11 09:53:20 GMT

How do we explain this? https://nostr.build/i/nostr.build_25af78a91cfc99f70fe9492ee09eafb5dd47f6f8f80ad98659a7bc5bbe1864fa.jpg

2023-04-10 01:22:32 GMT

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I think all the apps I use to check price have been down for like 3 weeks.

2023-04-09 01:51:22 GMT

#[0] recommend

2023-04-09 01:47:28 GMT

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I had one beer last night. It was a session beer. An hour after finishing it I had a splitting headache that stuck with me til the morning. It’s just not worth it these days. I have no tolerance anymore because I’m making better choices. The delta between waking up fresh and waking up hungover has never been wider.

2023-04-08 20:00:50 GMT

#[0] run

2023-04-08 10:29:49 GMT

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Congrats! My gigapet started sleeping through the night around that time, have you heard of dream feed?

2023-04-08 10:25:08 GMT

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How old?

2023-04-07 10:22:54 GMT

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The word “compete” hits different when the effort is decentralized as opposed to coordinated.

2023-04-05 09:51:11 GMT

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I had this. Lost my contact list. Couldn’t load any posts. Couldn’t add or delete relays. In Damus that nsec was bricked. Didn’t try in other clients.

2023-04-04 09:58:29 GMT

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Another vote for boulevardier, my personal favorite, followed by negronis (in the summer). Bother are bitter beasts

2023-04-03 16:27:43 GMT

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I’m looking forward to algorithms with parameters that I can control.

2023-04-03 10:20:58 GMT

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As an anon on here, I can tell you that I’ve already watched 2 of 3 recently released episode.

2023-04-02 19:56:58 GMT

#[0] can you list the top 5 nostr notes with the most zaps in the past 24 hours.

2023-04-02 18:57:57 GMT

In the market for some new kicks. Current set are All Birds. Love that they are lightweight and cleanable. They’ve also been going strong for nearly three years. Any recommendations?

2023-04-02 13:14:38 GMT

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Perfect

2023-04-02 12:49:09 GMT

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Only if my sats flow was strong. 10k btc might not be enough…

2023-04-02 12:45:06 GMT

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This was me 10 years ago. The lack of control caused my brain to run wild with all sorts of horrible scenarios. I think it was an early manifestation of “don’t trust verify”. I’ve come to think of it as a blessing and a curse.

2023-04-02 10:14:37 GMT

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Thanks for the love nostr! Looking forward to paying these zaps forward.
Also, I can’t get your DM out of “request”, seems like a glitch, maybe. Or user error, more probably.

2023-04-01 14:13:15 GMT

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3 eggs sunny side up in butter, whole milk yogurt with honey, a tangelo, coconut water, side of magnesium glycinate and D3

2023-04-01 11:01:04 GMT

#plebchain looking for a few follows to get going. I heard this was the place.

2023-04-01 10:56:54 GMT

Anyone here talking about science? Particularly biology related topics? I want to get in the conversation.

2023-04-01 10:54:55 GMT

Anyone one here talking about nutrition? Health? Any suggestions who to follow to get in the conversation or get it going?

2023-03-31 12:12:32 GMT

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Yes I totally agree that the authors did not resolve the discrepancies, of which I see two. The reference sample is supposed to be 70-80kDa and it’s >100 kDa. The sample also has two prominent bands one of which could plausibly be the correct mass given the low resolution of the gel and the shift in the recombinant control. Given that the detection antibody identifies both bands it is likely the smaller one is a truncated version of the other. Most likely a degradation product? That being said you’d need to do ALOT more work to show that.
The other issue is separate from this and I agree that reproducibility from lot to lot is paramount.

2023-03-31 10:25:52 GMT

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Hm, I agree that the reference protein and the sample don’t match, but they weren’t supposed to according to the figure legend. The reference sample does not contain the transmembrane domain of the drug substance and is lighter, which the blot confirms. That’s pretty much all you can take away from this. That and the expressed protein contains the same epitope that the reference sample contains for detection (despite different molecular weights)

2023-03-31 09:40:15 GMT

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Has someone been plotting this with time? The continuous grind higher is impressive.